3E5Z
X-Ray structure of the putative gluconolactonase in protein family PF08450. Northeast Structural Genomics Consortium target DrR130.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.420, 93.400, 76.990 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.010 |
| R-factor | 0.19466 |
| Rwork | 0.193 |
| R-free | 0.22908 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.087 | 0.368 |
| Number of reflections | 81184 | |
| <I/σ(I)> | 15.4 | 4.8 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M NaCl 0.1M TOPS 18% PEG 3350, MgL2 as additive, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
