3E5W

Crystal Structure Analysis of FP611

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-05-27
Detector	PSI PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	P 21 21 21
Unit cell lengths	71.250, 104.460, 133.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.800 - 1.710
R-factor	0.184
Rwork	0.183
R-free	0.20900
Starting model (for MR)	1uis
RMSD bond length	0.004
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.800	1.780
High resolution limit [Å]	1.710	1.710
Rmerge	0.075	0.748
Number of reflections	106080	11797
<I/σ(I)>	17.8	2.9
Completeness [%]	98.2	97.4
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	PEG 8000, pH 4.6, vapor diffusion, hanging drop, temperature 298K