3E5V
Crystal Structure Analysis of eqFP611 Double Mutant T122R, N143S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.536, 62.536, 208.022 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.890 - 2.100 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27800 |
| Starting model (for MR) | 1uis |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.742 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.074 | 0.454 |
| Number of reflections | 13944 | |
| <I/σ(I)> | 10.6 | 3.4 |
| Completeness [%] | 93.0 | 81.7 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
