3E5U
OCPA complexed CprK (C200S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.141, 117.170, 84.876 |
| Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
| Resolution | 19.210 - 1.830 |
| R-factor | 0.18467 |
| Rwork | 0.182 |
| R-free | 0.22664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.903 |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.210 |
| High resolution limit [Å] | 1.830 |
| Rmerge | 0.074 |
| Number of reflections | 88661 |
| <I/σ(I)> | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 200mM sodium chloride, 100mM sodium acetate, 20% PEG 6000, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
