3E5T
Crystal Structure Analysis of FP611
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.638, 69.416, 41.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.100 |
| Rwork | 0.116 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uis |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.048 | 0.247 |
| Number of reflections | 71928 | |
| <I/σ(I)> | 26 | 3 |
| Completeness [%] | 92.6 | 82.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 8000, pH 4.6, vapor diffusion, hanging drop, temperature 298K |
