3E58
Crystal structure of putative beta-phosphoglucomutase from Streptococcus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-12 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.784, 88.784, 118.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.860 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19907 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.080 | 0.404 |
| Number of reflections | 46069 | |
| <I/σ(I)> | 26.74 | 3.76 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M Lithium sulfate, 0.1M Tris-Cl pH 7.0, 2.0M Ammounium sulfate , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
