3E57
Crystal structure of Tm1382, a putative Nudix hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.589, 62.879, 73.896 |
| Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.890 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.216 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.890 | 4.090 | 1.900 |
| Rmerge | 0.059 | 0.038 | 0.387 |
| Number of reflections | 34267 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Trisodium Citrate pH 5.5, PEG 3000, vapor diffusion, hanging drop, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Trisodium Citrate pH 5.5, PEG 3000, vapor diffusion, hanging drop, temperature 298K |
