3E4R
Crystal structure of the alkanesulfonate binding protein (SsuA) from the phytopathogenic bacteria Xanthomonas axonopodis pv. citri bound to HEPES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-03-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.433 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.660, 85.500, 46.830 |
| Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
| Resolution | 46.370 - 2.010 |
| R-factor | 0.14545 |
| Rwork | 0.142 |
| R-free | 0.21975 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.374 | 2.120 |
| High resolution limit [Å] | 2.003 | 2.000 |
| Rmerge | 0.084 | 0.204 |
| Number of reflections | 15775 | |
| <I/σ(I)> | 4.72 | |
| Completeness [%] | 99.8 | 29.5 |
| Redundancy | 4.23 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES pH 7.5, 0.5 M Sodium Chloride, 1.6 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
