3E4G
Crystal structure of bovine coupling Factor B, G28E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.476, 50.010, 36.886 |
| Unit cell angles | 90.00, 109.37, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.960 |
| R-factor | 0.144 |
| Rwork | 0.143 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dze |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 0.985 |
| High resolution limit [Å] | 0.955 | 0.955 |
| Rmerge | 0.078 | |
| Number of reflections | 92547 | |
| <I/σ(I)> | 15.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 277 | 50% PPG 400, 100 mM Tris-HCl pH 7.4, VAPOR DIFFUSION, temperature 277K |
