3E4B
Crystal structure of AlgK from Pseudomonas fluorescens WCS374r
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.090, 107.820, 119.030 |
| Unit cell angles | 90.00, 96.97, 90.00 |
Refinement procedure
| Resolution | 49.050 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.11) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.546 |
| Number of reflections | 68665 | |
| <I/σ(I)> | 14.96 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.49 | 7.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 10% (w/v) PEG 6000, 0.1M MES pH 6.0, 0.56mM CYMAL-6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
