3E3M
Crystal structure of a LacI family transcriptional regulator from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.400, 60.443, 90.766 |
| Unit cell angles | 82.91, 76.96, 87.22 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.293 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.833 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | 0.502 |
| Total number of observations | 88241 | |
| Number of reflections | 156396 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 95.5 | 93.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 100mM Hepes pH 7.0, 20% PEG 3350, 200mM sodium chloride, Vapor diffusion, temperature 294K |
