3E35
Actinobacteria-specific protein of unknown function, SCO1997
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 135.083, 135.083, 135.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.200 |
| R-factor | 0.17747 |
| Rwork | 0.175 |
| R-free | 0.23401 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.895 |
| Number of reflections | 27763 | |
| <I/σ(I)> | 37.98 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.1 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1M MES pH 6.5, 0.55M Magnesium formate, 1.5% Glycerol, 0.25% N-octyl-beta-D-glucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
