3E2Y
Crystal structure of mouse kynurenine aminotransferase III in complex with glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.567, 91.567, 232.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.260 |
| R-factor | 0.17721 |
| Rwork | 0.175 |
| R-free | 0.22141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zjg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.830 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 45176 | |
| Completeness [%] | 95.4 | |
| Redundancy | 10.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 21% PEG 400, 150 mM CaCl2, 10% glycerol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
