3E2J
Crystal structure of bovine coupling factor B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.111 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.556, 133.941, 115.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.241 |
| Rwork | 0.237 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dze |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.746 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.076 | 0.581 |
| Number of reflections | 16810 | |
| Completeness [%] | 95.8 | 78.3 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 277 | 45% PPG 400, 100 mM Bis-Tris pH 6.5, pH 7.4, VAPOR DIFFUSION, temperature 277K |
