3E2D
The 1.4 A crystal structure of the large and cold-active Vibrio sp. alkaline phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.240, 165.980, 57.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.400 |
| R-factor | 0.15595 |
| Rwork | 0.155 |
| R-free | 0.16648 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.710 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.027 | 0.074 |
| Number of reflections | 205938 | |
| <I/σ(I)> | 17.8 | 10.9 |
| Completeness [%] | 92.9 | 68.8 |
| Redundancy | 4.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 277 | 0.2M Li2SO4, 0.1M Tris, 23% PEG 3350, 3% v/v ethylene glycol, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
