3DZT
AeD7-leukotriene E4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | MAR225 CCD |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.567, 65.307, 52.195 |
| Unit cell angles | 90.00, 112.34, 90.00 |
Refinement procedure
| Resolution | 32.650 - 1.800 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.20100 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.361 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | FFT (difference fourier) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.183 |
| Number of reflections | 28456 | |
| <I/σ(I)> | 10.25 | |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 16-20 % PEG 6000, 0.1 M Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
