3DZO
Crystal structure of a rhoptry kinase from toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.261, 44.288, 105.304 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.800 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3byv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.069 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 | 0.200 |
| Number of reflections | 34415 | |
| <I/σ(I)> | 173 | 4.23 |
| Completeness [%] | 97.6 | 81.4 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 22% PEG 4000, 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
