3DZ1
Crystal structure of Dihydrodipicolinate Synthase from Rhodopseudomonas palustris at 1.87A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.463, 84.264, 114.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.850 - 1.870 |
| R-factor | 0.21 |
| Rwork | 0.190 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.084 | 0.269 |
| Number of reflections | 26518 | |
| <I/σ(I)> | 16.1 | 12.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 13.9 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25%PEG 1,500 0.1M Bis-Tris pH 6.5 1.4% Butanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
