3DYP
E. coli (lacZ) beta-galactosidase (H418N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 96 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.380, 168.020, 200.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.700 - 1.750 |
| Rwork | 0.159 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.054 | 0.213 |
| Number of reflections | 486251 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 96.8 | 84.5 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
