3DY3
Crystal structure of yeast 20S proteasome in complex with the epimer form of spirolactacystin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-06-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.148, 301.860, 144.084 |
| Unit cell angles | 90.00, 112.81, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.810 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.850 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.094 | 0.467 |
| Number of reflections | 252667 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 98.2 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
