3DXY
Crystal structure of EcTrmB in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.040, 46.760, 58.130 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 17.900 - 1.500 |
| R-factor | 0.15526 |
| Rwork | 0.154 |
| R-free | 0.18508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dxx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.980 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 39292 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | 15% (w/v) PEG 3350, 150mM MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
