3DXS
Crystal structure of a copper binding domain from HMA7, a P-type ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.283 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.374, 44.529, 49.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.142 |
| Rwork | 0.142 |
| R-free | 0.16040 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.236 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.092 | 0.189 |
| Number of reflections | 7384 | |
| <I/σ(I)> | 23.6 | 7.4 |
| Completeness [%] | 90.0 | 82.3 |
| Redundancy | 6.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.2M LiCl, 0.1M Tris-HCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
