3DXP

Crystal structure of a putative aminoglycoside phosphotransferase (reut_a1007) from ralstonia eutropha jmp134 at 2.32 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97971,0.97956
Spacegroup name	P 21 21 2
Unit cell lengths	62.088, 127.522, 51.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.989 - 2.320
R-factor	0.238
Rwork	0.235
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.479
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.989	28.990	2.380
High resolution limit [Å]	2.320	10.380	2.320
Rmerge	0.129	0.059	0.718
Total number of observations		651	4674
Number of reflections	18395
<I/σ(I)>	5.3	6.9	1.1
Completeness [%]	99.9	95.4	100
Redundancy	3.5	2.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.17	293	20.0% polyethylene glycol 8000, 0.3M calcium acetate, 0.1M MES pH 6.17, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K