3DXF
Crystal structure of the HSCARG R37A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.450, 88.090, 80.201 |
| Unit cell angles | 90.00, 89.69, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.200 |
| R-factor | 0.24373 |
| Rwork | 0.242 |
| R-free | 0.28240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.087 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.700 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.212 |
| Number of reflections | 32788 | |
| <I/σ(I)> | 16.8 | 6.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 1.96M ammonium sulfate, 0.1M sodium Hepes pH 7.2, 2% MPD, 20mM potassium chloride, 5mM Phenol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
