3DX6

Crystal Structure of B*4402 presenting a 10mer EBV epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	50.696, 81.948, 109.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.650 - 1.701
R-factor	0.21
Rwork	0.208
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.206
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.650	1.745
High resolution limit [Å]	1.701	1.701
Number of reflections	45757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	Citrate, Ammonium Acetate, PEG 4000, pH 5.6, vapor diffusion, hanging drop, temperature 293K