3DX0
Golgi alpha-Mannosidase II in complex with Mannostatin A at pH 5.75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54182 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.853, 109.860, 139.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.570 - 1.700 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.560 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.054 | 0.330 |
| Number of reflections | 116271 | |
| <I/σ(I)> | 17.3 | 2.1 |
| Completeness [%] | 99.6 | 94.8 |
| Redundancy | 4.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG8000, Tris, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7 |
