3DWV
Glutathione peroxidase-type tryparedoxin peroxidase, oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.810, 105.864, 42.738 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.410 |
| R-factor | 0.128 |
| Rwork | 0.116 |
| R-free | 0.17640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gs3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.058 | 0.473 |
| Number of reflections | 71308 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 2M ammonium sulphate, 0.1M sodium acetate, 1mM EDTA, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
