3DWT

Structure of CabBCII-10 nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	76.720, 174.710, 115.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.245
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zmy
RMSD bond length	0.007
RMSD bond angle	1.523
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.127	0.485
Number of reflections	34579
<I/σ(I)>		2.7
Completeness [%]	99.9	100
Redundancy		6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1