3DWO
Crystal structure of a Pseudomonas aeruginosa FadL homologue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790, 1.0375, 1.1397 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.777, 233.054, 81.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25300 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.369 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.073 | 0.287 |
| Number of reflections | 28897 | |
| <I/σ(I)> | 10 | 2.4 |
| Completeness [%] | 89.7 | 38.6 |
| Redundancy | 5.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 28-32% PEG 400, 0.1M lithium sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
