3DWL
Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 218.967, 218.967, 315.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 3.780 |
| R-factor | 0.32469 |
| Rwork | 0.324 |
| R-free | 0.34367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k8k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.872 |
| High resolution limit [Å] | 3.780 | 3.775 |
| Number of reflections | 72502 | |
| <I/σ(I)> | 9.5 | 2.6 |
| Completeness [%] | 98.9 | 92.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 750 mM ammonium sulfate 50 mM sodium citrate 7 % glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
