3DW7
Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2656-SeCH3 modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-05-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.550, 29.550, 76.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.151 - 1.000 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.17000 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.100 | |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 66643 | 14613 |
| <I/σ(I)> | 15.24 | 2.5 |
| Completeness [%] | 95.6 | 84.1 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.2 M (NH4)2SO4, 50 mM K-MOPS pH 7.0, 10 mM MgCl2, 10 mM MnCl2, vapor diffusion, hanging drop, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | K-MOPS | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | (NH4)2SO4 | ||
| 7 | 1 | 2 | K-MOPS | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | MnCl2 |
