3DVV
Crystal structure of HIV-1 subtype F DIS extended duplex RNA bound to ribostamycin (U267OMe)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2007-07-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.703, 30.489, 58.004 |
Unit cell angles | 90.00, 122.25, 90.00 |
Refinement procedure
Resolution | 18.590 - 2.000 |
R-factor | 0.268 |
Rwork | 0.268 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3C3Z |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 9829 | |
<I/σ(I)> | 29 | 9.8 |
Completeness [%] | 95.6 | 91.3 |
Redundancy | 8.2 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MPD, cacodylate, MgCl2, KCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | cacodylate | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | cacodylate' | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | KCl |