3DVP
Pak1 peptide bound LC8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2007-11-01 |
| Detector | Onyx |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.404, 57.378, 64.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.650 - 2.500 |
| R-factor | 0.20687 |
| Rwork | 0.204 |
| R-free | 0.26283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.162 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0034) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 11.650 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 6394 |
| Completeness [%] | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 200 mM NaCl, 27% (w/v) PEG 4000, and 10% 0.1 M (NH4)6CoCl3, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K, temperature 293K |
