3DVE
Crystal Structure of Ca2+/CaM-CaV2.2 IQ domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 40.303, 40.303, 344.652 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.379 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.134 | 0.425 |
| Number of reflections | 7844 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 15 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1 M Bis-Tris, 25-30 % PEG 2000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
