3DVD
X-ray crystal structure of mutant N62D of human Carbonic Anhydrase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-25 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.772, 41.711, 72.831 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 21.360 - 1.600 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 31546 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 300 | 50mM Tris-HCl 1.3 M Sodium Citrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
