3DVD

X-ray crystal structure of mutant N62D of human Carbonic Anhydrase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	2008-03-25
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	42.772, 41.711, 72.831
Unit cell angles	90.00, 104.56, 90.00

Refinement procedure

Resolution	21.360 - 1.600
R-factor	0.179
Rwork	0.179
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	31546
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	300	50mM Tris-HCl 1.3 M Sodium Citrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 300K