3DV9
Putative beta-phosphoglucomutase from Bacteroides vulgatus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.247, 100.161, 38.991 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 26.900 - 1.720 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.900 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.093 | 0.576 |
| Number of reflections | 25739 | |
| <I/σ(I)> | 28.9 | 2.03 |
| Completeness [%] | 99.4 | 94.5 |
| Redundancy | 6.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.1 M magnesium formate, 15% PEG-3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
