3DV7
Role of Hydrophilic Residues in Proton Transfer During Catalysis by Human Carbonic Anhydrase II (N62A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-20 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.812, 41.774, 72.951 |
| Unit cell angles | 90.00, 104.62, 90.00 |
Refinement procedure
| Resolution | 19.040 - 1.700 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 25972 |
| Completeness [%] | 93.8 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 300 | 1.3 M Sodium Citrate, 50mM Tris-HCl, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
