3DU7
Tubulin-colchicine-phomopsin A: Stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-03 |
| Detector | ADSC |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 65 |
| Unit cell lengths | 327.126, 327.126, 53.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.100 |
| R-factor | 0.21781 |
| Rwork | 0.215 |
| R-free | 0.26471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Number of reflections | 25575 | |
| <I/σ(I)> | 12.2 | 1.9 |
| Completeness [%] | 96.0 | 90.8 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG, PIPES BUFFER. The crystal of tubulin-colchicine:RB3-SLD complex was soaked with a 0.7mM Phomopsin A solution for 24 hours., pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
