3DTJ
HIV-1 capsid C-terminal domain mutant (E187A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.603, 51.675, 51.797 |
| Unit cell angles | 109.94, 108.48, 110.02 |
Refinement procedure
| Resolution | 30.200 - 4.000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ds5 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.200 |
| High resolution limit [Å] | 4.000 |
| Rmerge | 0.092 |
| Number of reflections | 2160 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 62.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 298 | 32% PEG 4,000, 100 mM ammonium acetate pH 5.0 and 10 mM MgCl2., EVAPORATION, temperature 298K |
