3DTE
Crystal structure of the IRRE protein, a central regulator of DNA damage repair in deinococcaceae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.053, 52.813, 64.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21991 |
| Rwork | 0.218 |
| R-free | 0.25415 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.453 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 9581 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 281 | 20% PEG 3350, 0.2M Potassium Fluoride, pH 7.5, VAPOR DIFFUSION, temperature 281K |
