3DSC
Crystal structure of P. furiosus Mre11 DNA synaptic complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 98.477, 106.071, 76.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.700 |
| R-factor | 0.23034 |
| Rwork | 0.228 |
| R-free | 0.27804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.085 |
| Number of reflections | 10769 |
| <I/σ(I)> | 17.6 |
| Completeness [%] | 94.5 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 1000, 0.1 M Tris-HCl, 0.2 M MgCl2, 0.2 M 1,6 hexanediol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 1000 | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | 1,6 hexanediol | ||
| 5 | 1 | 2 | PEG 1000 | ||
| 6 | 1 | 2 | Tris | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | 1,6 hexanediol |
