3DS2
HIV-1 capsid C-terminal domain mutant (Y169A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.670, 75.660, 32.870 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 27.270 - 1.200 |
| R-factor | 0.19249 |
| Rwork | 0.191 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2buo |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.201 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.041 | 0.361 |
| Number of reflections | 38077 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 85.5 | 43.5 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 20% PEG 3350, 200mM sodium iodide, pH 7.5, EVAPORATION, temperature 298.0K |
