3DRN
The crystal structure of Bcp1 from Sulfolobus Sulfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.841, 91.829, 52.542 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| Rwork | 0.189 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a4v |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 21792 | |
| Completeness [%] | 98.0 | 82.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.25M ammonium acetate, 24% PEG 4000, 0.1M Sodium citrate buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
