3DR6
Structure of yncA, a putative ACETYLTRANSFERASE from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.898, 70.229, 59.157 |
| Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
| Resolution | 39.750 - 1.750 |
| R-factor | 0.16489 |
| Rwork | 0.163 |
| R-free | 0.20012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DR8 chain A |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.386 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | |
| Number of reflections | 61706 | |
| <I/σ(I)> | 25.4 | 5.6 |
| Completeness [%] | 93.9 | 67.1 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20% PEG 5K MME, 0.1 M Bis-Tris, cryoprotected with Paratone-N oil, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
