3DQX
chicken c-Src kinase domain in complex with ATPgS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.507, 119.150, 63.676 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 34.610 - 2.300 |
| R-factor | 0.19385 |
| Rwork | 0.191 |
| R-free | 0.24131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2SRC with deletion of residues 300-315 and 400-425 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.205 |
| Number of reflections | 27164 | |
| <I/σ(I)> | 18 | 4.49 |
| Completeness [%] | 96.6 | 81.4 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% PEG 4000, 50 mM Ammonium acetate, 100 mM Bis-Tris pH 5.5, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
