3DQP
Crystal structure of the oxidoreductase ylbE from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR121.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97862 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.567, 34.790, 66.379 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 9.840 - 1.400 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.16700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.234 |
| Number of reflections | 78964 | |
| <I/σ(I)> | 23.6 | 4.59 |
| Completeness [%] | 90.4 | 99.7 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, under oil | 7.5 | 291 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 0.1M HEPES (pH 7.5), 40% PEG 20K, and 0.1M NaMolybdate., microbatch, under oil, temperature 291K |
