3DQ0

Maize cytokinin oxidase/dehydrogenase complexed with N6-(3-methoxy-phenyl)adenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	252.700, 50.400, 51.000
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	46.180 - 1.900
R-factor	0.207
Rwork	0.204
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bw7
RMSD bond length	0.008
RMSD bond angle	1.099
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.043	0.159
Number of reflections	48203	6334
<I/σ(I)>	17.95	7
Completeness [%]	94.7	87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15% PEG 1500, 0.5% n-octyl beta-D-glucoside, Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K