3DOW
Complex structure of GABA type A receptor associated protein and its binding epitope on calreticulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93400 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 97.043, 97.043, 97.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.310 - 2.300 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kjt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.620 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.620 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.399 |
| Total number of observations | 6540 | |
| Number of reflections | 6892 | |
| <I/σ(I)> | 11.1 | 1.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M MES, 27% v/v PEG MME 550, 10 mM ZnSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
