3DOB
Peptide-binding domain of Heat shock 70 kDa protein F44E5.5 from C.elegans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 102.009, 102.009, 80.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.800 - 2.390 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2op6.pdb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.400 |
| Rmerge | 0.079 | 0.905 |
| Number of reflections | 19448 | |
| <I/σ(I)> | 10.8 | 2.85 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.9 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 277 | 50 mM Tris buffer, 0.8 M ammonium sulfate, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
