3DO8
The crystal structure of the protein with unknown function from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.001, 63.781, 47.047 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 47.890 - 1.600 |
| R-factor | 0.16909 |
| Rwork | 0.167 |
| R-free | 0.21372 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.059 | 0.331 |
| Number of reflections | 34228 | |
| <I/σ(I)> | 25.78 | 2.25 |
| Completeness [%] | 96.2 | 86.87 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M Hepes, pH7.5, 30%PEG MME, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
